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A Pharisee was a member of a sect of the Jewish religion in the time of Christ characterized by an encyclopedic knowledge of scripture and a fixation with the minutiae of said scripture as a means of understanding God's will. For example the scripture required tithing--giving 10% of income, or yearly increase--to the priesthood. Pharisees went to the trouble of counting the seeds and leaves of the plants in their garden and tithing on those amounts.
The Pharisees also emphasized strong moral ideals, some of which would be seen as impractical to most people. Most of these are laid out in the section of the Talmud known as Pirke Avot.
The Christian Bible describes them in invariably negative terms. According to Christian tradition, the Pharisees sought harsh penalties for the smallest of infractions, and, like all fanatics, considered themselves far more important than everyone else. As a result, the term "Pharisee" is generally an insult in Christian cultures, generally meaning a hypocritical, self-righteous nitpicker.
However, the Pharisees were also one of the most prominent sects of Judaism due to their emphasis on scriptural knowledge and interpretation, and were also the ones most directly responsible for the creation of the immense body of theological commentary known as the Talmud (as well as rabbinical Judaism as a whole) as a way to preserve Jewish identity after the destruction of the Second Temple and the last vestiges of the Judahite temple-state. The traditional Jewish emphasis on learning and study from medieval to modern times is therefore a direct positive legacy of Pharisaic influence.