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Glenn was raised Roman Catholic, and was still a Christian when she started vlogging on YouTube. In her early videos, she generally expressed liberal views on social matters. She apparently began to question Catholicism's stance on divorce, which led to a questioning of her faith more generally. In 2012, she revealed that she was no longer religious, and now identified as an agnostic. She has since come to call herself an atheist.
She is opposed to Atheism+, which she describes as "atheism plus radical feminism". Despite this definition being far from the truth, she seems to support a lot of the same values as Atheism+ at times.
Glenn is an outspoken supporter of gay and lesbian rights but has espoused views on her YouTube channel which are considered by some to be trans-exclusionary. Specifically she has said that a trans man who presented as a man but had female genitalia was "biologically, a woman"  thus de-legitimizing the identity of any trans person yet to have gender-affirming surgery. Glenn teamed up with self-admitted transphobe Arielle Scarcella to attack Riley Dennis and her girlfriend Fiona to the point where Fiona decided to shut down the channel. Glenn later defended the video, this time going one step further by saying she would not date a man she otherwise found attractive specifically because he was trans.  Glenn had earlier collaborated with Scarcella to launch an attack on non-binary, intersex, and gender queer persons in her infamous Two Genders video. 
Relationship with feminism
Glenn has attracted a fair number of MRAs to her videos after voicing her opinions against feminism. It seems her main issues with feminism are radical feminists. Some have criticized her for being an "MRA apologist". Some radical feminists have also criticized her for posing for Playboy.
Following the massacre committed by Elliot Rodger, she made a video arguing that Rodger's actions were solely based on mental illness, rather than misogyny. She has even gone so far as to claim feminists were "hijacking" the massacre to make it look like it was influenced by misogyny, when, according to Glenn, it wasn't. Glenn's view specifically was endorsed by Dawkins. Never mind that Rodger himself, widely considered one of the foremost experts on his motives, pretty explicitly debunked this.
Glenn came under fire in 2015 for allegations of plagiarism. The controversy began when she was called on a video she made about Kim Davis, in which she repeated every word of a video by Theoretical Bullshit. She was then caught also reading word for word the comments of her Facebook fans and passing them off as her own. Afterwards, other videos of hers were found to be copied dialogue of other individuals, including Sam Harris. However, she mostly plagiarized — that is, copied verbatim without attribution — comments on her Facebook and dialogue from other YouTube videos. Many other YouTubers condemned her actions, including her buddy TheAmazingAtheist. She has since given a half-assed apology admitting she did some wrong doing, but not really. Interestingly enough she hasn't even been prudent enough to delete the evidence of her plagiarism from her Facebook page which is available to see.
- JaclynGlenn - About. YouTube.
- Atheism + Drama
- YouTube JaclynGlenn. Glenn responding to a video by trans activist Riley Dennis.
- YouTube JaclynGlenn. Jaclyn defends video collaboration with Arielle Scarcella, mocks Riley Dennis and NeonFiona.
- YouTube JaclynGlenn. Two Genders video.
- Twitter "Yes, I am an antifeminist. It's not that I'm calling out the behavior of some social justice warriors, no. It's b/c I just hate women. Duh." Sarcasm included.
- A response to Jaclyn Glenn's Elliot Rodger video
- Butterflies & Wheels on Freethought Blogs.
- Screenshot backup
- You decide.
- Jaclyn Glenn Plagiarizing Sam Harris
- Jaclyn Glenn addresses the accusations of plagiarism. The video also outlines some of her views on transphobia and feminism.